Figure 2: NE and Ach at doses of 0.1, 1, 10, and 100 μM on the viability of Leydig cells from mice on PD15. The viability of Leydig cells incubated with different doses of NE and Ach for 24 h was assessed using MTT assays. The data were presented as means ± SEM. A single asterisks (*) indicates statistical significance () relative to the untreated control. CON: control group cultured with the same dilution of NE and Ach. hCG: hCG group cultured with hCG at the dose of 1 IU/mL.