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Figure 5: Expression levels of 3β-HSD mRNA and PCNA mRNA in the cultured adult Leydig cells and MLTC-1 treated with NE, and Ach. Asterisks (*) indicate statistical significance ( 𝑃 < 0 . 0 5 ) between the CON, NE and Ach groups. CON: Control group cultured with the same dilution of NE and Ach. NE: NE group cultured in the presence of 10 μM NE. Ach: Ach group cultured in the presence of 10 μM Ach.