Troglitazone Induces Extracellular Matrix and Cytoskeleton Remodeling in Mouse Collecting Duct Cells
Figure 1
PPARγ is present in the IMCD-K2 and M1 collecting duct cell lines. (a) Western blot analysis of PPARγ detected a band at 67 kDa in IMCDK2 cells, also shown in mouse cortex and medulla lysates used as a positive control. (b) In M1 cells, endogenous PPARγ levels as well as those of the pcDNA plasmid control and pcDNA-PPARγ overexpression samples are shown, included for each are samples treated for 24 hours with DMSO (vehicle) or 10 μM troglitazone (TRO). (c) In different passages of M1 cells (ctr, P33, 46, and 48), RT-PCR analysis produced a 400 bp product corresponding to PPARγ detected on 1% agarose gel. The (RT-) samples were used as a negative control. Lanes identified as L contain DNA ladder.
