Ultrasound Biomicroscopy in Small Animal Research: Applications in Molecular and Preclinical Imaging

Figure 7

In utero microinjection: laparoscopy along the linea alba. The uterine horns are gently exteriorized to record implantation sites and simply positioned on sterile gauze and covered with sterile acoustic gel (a). A 40 Mhz probe is used to image in real time the embryos, and the microinjection is performed by an automatic microinjector equipped with a capillary glass needle. The needle is advanced with the use a micromanipulator under echo guidance until the needle tip is in the desired location (b). UBM images of a capillary glass needle advanced under echo guidance until the needle tip is in the amnions (c).