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Figure 2: (a) The PKCδ-specific inhibitor Rottlerin inhibited 10% CSE-induced cell proliferation of rPASMCs. The rPASMCs were treated with different concentrations of Rottlerin for 1 h before exposure to 10% CSE. Cell proliferation was analyzed by MTT assay. The data are shown as mean ± standard deviation from 6 experiments and were analyzed by ANOVA. * 𝑃 < 0 . 0 5 compared with control and # 𝑃 < 0 . 0 5 compared with 10% CSE. (b) The PKCδ-specific inhibitor Rottlerin inhibited the CSE-induced cell cycle progression of rPASMCs. The rPASMCs were treated with different concentrations of Rottlerin for 1 h before exposure to 10% CSE. Cells were prepared and stained with propidium iodide solution for cell cycle assay. S phase and G2 phase cells were averaged. The data are shown as mean ± standard deviation from 6 experiments and were analyzed by ANOVA. * 𝑃 < 0 . 0 5 compared with control and # 𝑃 < 0 . 0 5 as compared with 10% CSE. (c) The PKCδ-specific inhibitor Rottlerin inhibited the CSE-induced upregulation of the mRNA and protein levels of PDGF signaling pathway. The rPASMCs were treated with different concentrations of Rottlerin for 1 h before exposure to 10% CSE. Cell extracts were prepared and submitted to RT-PCR or immunoblot assay with primers or antibodies as indicated.