BioMed Research International

Research Article

Nuclear Expression of a Mitochondrial DNA Gene: Mitochondrial Targeting of Allotopically Expressed Mutant ATP6 in Transgenic Mice

Table 1

Motor and biochemical analyses of A6M and A6W transgenic mice. Hazard and odds ratios are expressed with 95% confidence intervals (95% CI) and 𝑃 values. Biochemical data are expressed as mean ± standard error of the mean (SE).
A6MA6W
Wirehang hazard ratio (95% CI)HR: 0.54 (0.376–0.770)HR: 0.424 (0.246–0.731)
𝑃 = 0 . 0 0 0 8 𝑃 = 0 . 0 0 2
BB slips/crossing odds ratio (95% CI)OR: 0.12 (0.021–0.709)OR: 0.68 (0.141–3.276)
𝑃 = 0 . 0 1 9 1 𝑃 = 0 . 6 3 0 5
BB time hazard ratio (95% CI)HR: 1.72 (1.274–2.309)HR: 0.67 (0.506–0.896)
𝑃 = 0 . 0 0 0 4 𝑃 = 0 . 0 0 6 7
Constant Rota-Rod hazard ratio (95% CI)HR: 2.28 (1.722–3.030)HR: 2.09 (1.414–3.097)
𝑃 < 0 . 0 0 0 1 𝑃 = 0 . 0 0 0 2
Accelerating Rota-Rod hazard ratio (95% CI)HR: 2.37 (1.589–3.523)HR: 1.09 (0.681–1.739)
𝑃 < 0 . 0 0 0 1 𝑃 = 0 . 7 2
Poletest hazard ratio (95% CI)HR: 1.50 (1.156–1.941)HR: 1.10 (0.860–1.399)
𝑃 = 0 . 0 0 2 2 𝑃 = 0 . 4 6
Gait
LF-LF 𝑃 = 1 . 0 𝑃 = 1 . 0
LF-LR 𝑃 = 1 . 0 𝑃 = 1 . 0
LF-RF 𝑃 = 1 . 0 𝑃 = 1 . 0
LR-LR 𝑃 = 1 . 0 𝑃 = 1 . 0
LR-RR 𝑃 = 1 . 0 𝑃 = 1 . 0
RF-RF 𝑃 = 1 . 0 𝑃 = 1 . 0
RF-RR 𝑃 = 1 . 0 𝑃 = 1 . 0
RR-RR 𝑃 = 0 . 3 6 𝑃 = 1 . 0
Brain state II (nmol O2/min/mg p r o t e i n ± S E )Transgenic: 1 0 . 1 ± 1 . 2 Transgenic: 7 . 5 ± 0 . 7
Nontransgenic: 8 . 9 ± 1 . 9 Nontransgenic: 8 . 5 ± 0 . 8
𝑃 = 0 . 6 0 9 4 𝑃 = 0 . 3 1 4 9
Heart state II (nmol O2/min/mg p r o t e i n ± S E )Transgenic: 2 0 . 2 ± 1 . 3 Transgenic: 2 1 . 1 ± 1 . 3
Nontransgenic: 2 1 . 5 ± 1 . 6 Nontransgenic: 2 1 . 1 ± 1 . 2
𝑃 = 0 . 5 2 9 1 𝑃 = 0 . 9 6 3 4
Skeletal muscle state II (nmol O2/min/mg p r o t e i n ± S E )Transgenic: 1 4 . 5 ± 1 . 7 Transgenic: 1 5 . 4 ± 1 . 3
Nontransgenic: 1 6 . 3 ± 1 . 8 Nontransgenic: 1 4 . 6 ± 1 . 2
𝑃 = 0 . 4 6 7 2 𝑃 = 0 . 6 6 8 7
Brain state III (nmol O2/min/mg p r o t e i n ± S E )Transgenic: 2 4 . 2 ± 2 . 7 Transgenic: 2 0 . 3 ± 1 . 1
Nontransgenic: 2 3 . 1 ± 1 . 2 Nontransgenic: 2 4 . 0 ± 2 . 2
𝑃 = 0 . 7 1 2 8 𝑃 = 0 . 1 5 0 8
Heart state III (nmol O2/min/mg p r o t e i n ± S E )Transgenic: 3 1 . 4 ± 2 . 0 Transgenic: 3 1 . 6 ± 2 . 1
Nontransgenic: 3 3 . 1 ± 3 . 0 Nontransgenic: 3 2 . 8 ± 1 . 9
𝑃 = 0 . 5 9 6 5 𝑃 = 0 . 6 9 1 0
Skeletal muscle state III (nmol O2/min/mg p r o t e i n ± S E )Transgenic: 3 5 . 1 ± 3 . 5 Transgenic: 3 4 . 4 ± 3 . 7
Nontransgenic: 3 4 . 7 ± 4 . 0 Nontransgenic: 3 1 . 0 ± 2 . 6
𝑃 = 0 . 9 4 3 8 𝑃 = 0 . 5 8 8 2
Brain respiratory control ratioTransgenic: 2 . 4 8 ± 0 . 1 8 Transgenic: 2 . 8 4 ± 0 . 2 1
Nontransgenic: 3 . 1 9 ± 0 . 4 1 Nontransgenic: 3 . 0 1 ± 0 . 4 1
𝑃 = 0 . 1 4 5 8 𝑃 = 0 . 7 1 9 2
Heart respiratory control ratioTransgenic: 1 . 5 7 ± 0 . 0 8 Transgenic: 1 . 5 1 ± 0 . 0 6
Nontransgenic: 1 . 5 3 ± 0 . 0 5 Nontransgenic: 1 . 5 8 ± 0 . 1 1
𝑃 = 0 . 6 7 0 7 𝑃 = 0 . 5 5 3 0
Skeletal muscle respiratory control ratioTransgenic: 2 . 5 9 ± 0 . 2 6 Transgenic: 2 . 2 4 ± 0 . 1 7
Nontransgenic: 2 . 1 6 ± 0 . 1 2 Nontransgenic: 2 . 2 0 ± 0 . 1 1
𝑃 = 0 . 1 5 9 5 𝑃 = 0 . 8 7 0 9
Brain ATP synthesis (nmol/min/mg protein)Transgenic: 6 0 ± 2 . 0 Transgenic: 6 5 ± 7 . 9
Nontransgenic: 6 4 ± 6 . 7 Nontransgenic: 6 2 ± 4 . 9
𝑃 = 0 . 5 9 9 1 𝑃 = 0 . 7 1 4 3
Heart ATP synthesis (nmol/min/mg protein)Transgenic: 6 4 ± 1 0 . 8 Transgenic: 6 8 ± 8 . 3
Nontransgenic: 5 8 ± 5 . 7 Nontransgenic: 6 5 ± 4 . 7
𝑃 = 0 . 6 0 0 6 𝑃 = 0 . 7 5 4 9
Skeletal muscle ATP synthesis (nmol/min/mg protein)Transgenic: 3 6 ± 2 . 9 Transgenic: 3 8 ± 2 . 1
Nontransgenic: 4 0 ± 3 . 6 Nontransgenic: 3 7 ± 2 . 3
𝑃 = 0 . 4 1 6 7 𝑃 = 0 . 6 9 0 2
Brain MnSOD protein levels (arbitrary colorimetric values)Transgenic: 4 . 6 ± 0 . 6 6 Transgenic: 5 . 4 ± 1 . 1 1
Nontransgenic: 5 . 2 ± 0 . 4 7 Nontransgenic: 4 . 2 ± 0 . 3 9
𝑃 = 0 . 4 0 9 8 𝑃 = 0 . 3 5 1 4
Heart MnSOD protein levels arbitrary colorimetric values)Transgenic: 3 . 7 ± 0 . 1 1 Transgenic: 4 . 6 ± 0 . 3 3
Nontransgenic: 4 . 0 ± 0 . 1 5 Nontransgenic: 4 . 2 ± 0 . 1 3
𝑃 = 0 . 2 6 9 1 𝑃 = 0 . 2 5 6 9
Skeletal muscle MnSOD protein levels (arbitrary colorimetric values)Transgenic: 5 . 1 ± 0 . 9 3 Transgenic: 3 . 9 ± 0 . 3 5
Nontransgenic: 5 . 0 ± 0 . 5 7 Nontransgenic: 3 . 9 ± 0 . 4 6
𝑃 = 0 . 9 3 6 1 𝑃 = 0 . 9 5 9 0
Serum lactate (mM)Transgenic: 1 1 . 2 ± 0 . 8 3 Transgenic: 9 . 6 ± 0 . 5 1
Nontransgenic: 1 2 . 5 ± 0 . 8 3 Nontransgenic: 1 1 . 6 ± 0 . 7 3
𝑃 = 0 . 2 0 7 3 𝑃 = 0 . 0 3 7 0
LF: left front; LR: left rear; RF: right front; RR: right rear.