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Figure 3: Immunofluorescence characterization of hAMSCs. Representative images of fluorescence microscopy staining. The upper left panel (denoted as “negative”) shows cells incubated with the secondary antibody only. hAMSCs were positive for CD29, CD90, fibronectin, vimentin, and CK 18 and negative for ZO-1 and CK 7. Nuclei were counterstained with DAPI. Original magnification 20x.