Amniotic Mesenchymal Stem Cells: A New Source for Hepatocyte-Like Cells and Induction of CFTR Expression by Coculture with Cystic Fibrosis Airway Epithelial Cells

Table 3

Percentages of CFTR⁺ hMSCs labelled with CM-DiI in cocultures with CFBE41o- cells.

	% of CM-DiI^+ CFTR^+ cells	% of CFTR^+ in whole CM-DiI^+ population	𝑃
hAMSCs	—	6.2±2.0	—
CFBE	—	11.2±1.3	0.0006
hAMSC-CFBE 1 : 5	12.1±2.5	50.0±6.1	<0.0001
hAMSC-CFBE 1 : 10	7.5±2.1	46.7±9.3	<0.0001
hAMSC-CFBE 1 : 15	3.0±0.4	33.2±6.5	<0.0001
hAMSC-CFBE 1 : 20	2.2±0.9	34.6±8.7	<0.0001

Percentages of CM-DiI⁺ CFTR⁺ cells were obtained by plotting the FLH-1 channel, identifying CFTR-specific green signal, against FLH-2 channel, identifying red-labelled hAMSCs. Percentages of CFTR-expressing hAMSCs in whole CM-DiI⁺ population were obtained by dividing the double positive hAMSCs for all CM-DiI⁺ cells (with and without green signal). Data are shown as the mean ± SD of five experiments. Significance is referred to CFTR⁺ cells in the whole CM-DiI⁺ population in all conditions as compared with hMSCs alone.