Table 3: Percentages of CFTR+ hMSCs labelled with CM-DiI in cocultures with CFBE41o- cells.

% of CM-DiI+ CFTR+ cells% of CFTR+ in whole CM-DiI+ population 𝑃

hAMSCs 6 . 2 ± 2 . 0
CFBE 1 1 . 2 ± 1 . 3 0.0006
hAMSC-CFBE 1 : 5 1 2 . 1 ± 2 . 5 5 0 . 0 ± 6 . 1 <0.0001
hAMSC-CFBE 1 : 10 7 . 5 ± 2 . 1 4 6 . 7 ± 9 . 3 <0.0001
hAMSC-CFBE 1 : 15 3 . 0 ± 0 . 4 3 3 . 2 ± 6 . 5 <0.0001
hAMSC-CFBE 1 : 20 2 . 2 ± 0 . 9 3 4 . 6 ± 8 . 7 <0.0001

Percentages of CM-DiI+ CFTR+ cells were obtained by plotting the FLH-1 channel, identifying CFTR-specific green signal, against FLH-2 channel, identifying red-labelled hAMSCs. Percentages of CFTR-expressing hAMSCs in whole CM-DiI+ population were obtained by dividing the double positive hAMSCs for all CM-DiI+ cells (with and without green signal). Data are shown as the mean ± SD of five experiments. Significance is referred to CFTR+ cells in the whole CM-DiI+ population in all conditions as compared with hMSCs alone.