Camel Milk Triggers Apoptotic Signaling Pathways in Human Hepatoma HepG2 and Breast Cancer MCF7 Cell Lines through Transcriptional Mechanism
Figure 3
Effect of camel milk on oxidative stress markers HO-1 (a) and NQO1 (b) mRNA levels, and ROS (c) production in HepG2 cells. (a) and (b) HepG2 cells were treated for 6 h with a various concentrations of camel milk (2.5, 5, 10, and 20 mg/mL). Thereafter, total RNA was isolated using TRIzol reagent and the mRNA levels of HO-1 and NQO1 were quantified using RT-PCR normalized to β-actin housekeeping gene as described Section 2. Duplicate reactions were performed for each experiment, and the values presented are the means ± SEM () of three independent experiments. compared with untreated cells. (c) HepG2 cells were treated for 24 h with a various concentrations of camel milk (2.5, 5, 10, and 20 mg/mL). Thereafter, cells were incubated with DCF-DA (10 μM) for 1 h. DCF formation was measured fluorometrically using excitation/emission wavelengths of 484/535 nm. Values are presented as means ± SEM, . , compared to control (0 mg/mL).
