GFP::DAO-5 expression in worms and HeLa cells. (a) A transgenic worm expressing GFP::DAO-5 shows an oval distribution of green fluorescence representing cell nucleoli. (b and c) HeLa cells transfected by plasmids containing gfp::dao-5 and co-stained with anti-fibrillarin antibody show the location of nucleoli expressing GFP fusion protein (arrows). (d) The same field of cells stained by a DNA dye and visualized through a UV fluorescence microscope. The scale bar in (a) indicates 100 μm.
