Research Article

Distribution of Genes and Repetitive Elements in the Diabrotica virgifera virgifera Genome Estimated Using BAC Sequencing

Table 2

Summary of contigs per BAC that were assembled from Roche-454 sequencing data.

Mid-tag libraryBAC cloneMarkerRaw data (reads/kb)Assembled data (reads/kb)GenBank accessionContig size (kb)

RL00140F02130423,300/10,298 21,525/97.8JQ581035 1 9 . 6 ± 2 2 . 2
RL00289B101224 8,701/7,408 7,277/118.7JQ581036 2 9 . 7 ± 2 0 . 6
RL003142B02120329,444/12,894 19,447/30.5JQ581037 4 . 3 ± 3 . 6
RL005191G22112564,410/28,909 8,348/104.0JQ581038 1 7 . 3 ± 1 8 . 8
RL006163F141304 9,495/3,523 22,530/101.7JQ581039 2 5 . 4 ± 7 . 2
RL007156M20120316,427/7,179 9,435/24.6JQ581040 4 . 1 ± 2 . 8
RL008222P02134543,702/20,431 0/0.0FAILEDNA
RL009213A05141125,196/11,345 18,868/74.5JQ581041 7 4 . 5 ± 0 . 0
RL010188M01130019,912/9,238 18,478/90.6JQ581042 1 5 . 1 ± 9 . 7

Total240,587/111,225 125,908 (15,738 ± 6286)/642.3 ( 8 0 . 3 ± 3 4 . 9 ) 1 3 . 6 ± 2 0 . 1

NA: not applicable due to DNA sequencing failure.