Actinobacillus succinogenes ATCC 55618 Fermentation Medium Optimization for the Production of Succinic Acid by Response Surface Methodology

Table 2

Experiment design and results of the steepest ascent path.

Runs	Glucose (g L^−1)	Yeast extract (g L^−1)	MgCO3 (g L^−1)	OD660	Succinic acid production (g L^−1)
1	80	10	60	6.1 ± 0.2^a	48.4 ± 1.3
2	85	7	65	3.7 ± 0.7	41.7 ± 2.6
3	90	4	70	0.6 ± 0.0	36.4 ± 2.4
4	95	1	75	0.2 ± 0.0	22.1 ± 5.3
5	100	0	80	0.1 ± 0.0	4.2 ± 1.1

^aStandard error was calculated from three independent samples.