High-Resolution Whole-Mount In Situ Hybridization Using Quantum Dot Nanocrystals
Figure 4
Double whole-mount in situ hybridization against cardiac actin and Xa-1 and the intracellular distribution of LTBP1 and Xa1. (a) A comparison of the staining pattern between the chromogenic and QD-based visualization of the FITC-labeled Xa-1 probe, shown in red, reveals that the QD staining is identical to that obtained using the standard chromogenic protocol. (b) An embryo processed using Biotin and FITC-labeled probes against cardiac actin (green) and Xa-1 (red), respectively. The two probes were visualized with spectrally resolvable QDs demonstrating that two color fluorescent in situs can be performed using QDs. The inset shows the chromogenic staining for cardiac actin for comparison. (c, d) Images of embryos processed for whole-mount in situ hybridization and counterstained with Hoechst (blue) at 20X (c) and 40X (d) magnification, showing differences in the intracellular distribution of the transcripts of Xa-1 (c) and LTBP1 (d), both shown in red.
