High-Resolution Whole-Mount In Situ Hybridization Using Quantum Dot Nanocrystals

Table 1

Comparative chart of different permeabilization approaches used in whole mount immunostaining using a biotinylated anti-phosphotyrosine antibody (4G10 clone) and detected with Streptavidin conjugated QDs. Proteinase K treatment is the only approach which allowed deep tissue staining using QDs.

	Superficial Staining	Deep Tissue Staining
TRITON 1%	+++	−
TRITON 5%	+++	−
0.2% SDS 2 h	+++	+
0.2% SDS 4 h	+++	+
PK 25 min	+++	+++