639562.fig.002a
(a)
639562.fig.002b
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639562.fig.002c
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639562.fig.002d
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Figure 2: (a) Cellular uptake of 131I-SHH in breast cancer cell lines at one hour. Data is represented as the % injected dose (cpm (cells)/cpm (media))/mg protein. (b) Western blot showing expression of PTCH-1 in breast cancer cell lines. Cellular uptake of 131I-SHH is correlated with expression of the PTCH-1 receptor. (c) Nuclear Gli-1 expression in breast cancer cell lines. Data is expressed as number of cells with intense nuclear Gli-1 staining. (d) Time course showing increased accumulation of 131I-SHH in SKBR3 cancer cells with time. Uptake was blocked by 100-fold excess cold SSH.