Detection of Canonical Hedgehog Signaling in Breast Cancer by 131-Iodine-Labeled Derivatives of the Sonic Hedgehog Protein

Figure 2

(a) Cellular uptake of ¹³¹I-SHH in breast cancer cell lines at one hour. Data is represented as the % injected dose (cpm (cells)/cpm (media))/mg protein. (b) Western blot showing expression of PTCH-1 in breast cancer cell lines. Cellular uptake of ¹³¹I-SHH is correlated with expression of the PTCH-1 receptor. (c) Nuclear Gli-1 expression in breast cancer cell lines. Data is expressed as number of cells with intense nuclear Gli-1 staining. (d) Time course showing increased accumulation of ¹³¹I-SHH in SKBR3 cancer cells with time. Uptake was blocked by 100-fold excess cold SSH.

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