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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 647265, 10 pages
http://dx.doi.org/10.1155/2012/647265
Research Article

Self-Organization of Motor-Propelled Cytoskeletal Filaments at Topographically Defined Borders

1School of Natural Sciences, Linnaeus University, 391 82 Kalmar, Sweden
2Division of Solid State Physics and The Nanometer Structure Consortium, Lund University, Box 118, S221 00 Lund, Sweden

Received 31 August 2011; Accepted 7 January 2012

Academic Editor: P. Bryant Chase

Copyright © 2012 Alf Månsson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Self-organization phenomena are of critical importance in living organisms and of great interest to exploit in nanotechnology. Here we describe in vitro self-organization of molecular motor-propelled actin filaments, manifested as a tendency of the filaments to accumulate in high density close to topographically defined edges on nano- and microstructured surfaces. We hypothesized that this “edge-tracing” effect either (1) results from increased motor density along the guiding edges or (2) is a direct consequence of the asymmetric constraints on stochastic changes in filament sliding direction imposed by the edges. The latter hypothesis is well captured by a model explicitly defining the constraints of motility on structured surfaces in combination with Monte-Carlo simulations [cf. Nitta et al. (2006)] of filament sliding. In support of hypothesis 2 we found that the model reproduced the edge tracing effect without the need to assume increased motor density at the edges. We then used model simulations to elucidate mechanistic details. The results are discussed in relation to nanotechnological applications and future experiments to test model predictions.