648170.fig.003a
(a)
648170.fig.003b
(b)
648170.fig.003c
(c)
Figure 3: T3 selectively antagonizes Sp1 but not NF-κB-dependent transcription of MUC5AC in HBE16 cells. (a) After the cells were cotransfected with Sp1 expression plasmid, the luciferase activity was determined as described in Materials and Methods. Blank: different MUC5AC promoter plasmids only; control: different MUC5AC promoter plasmids plus Sp1 expression plasmid in the absence of T3; experimental: different MUC5AC promoter plasmids plus Sp1 expression plasmid in the presence of T3. 𝑃 > 0 . 0 5 versus blank; 𝑃 < 0 . 0 1 versus blank; # 𝑃 > 0 . 0 5 versus control; ## 𝑃 < 0 . 0 1 versus control. (b) After the cells were cotransfected with NF-κB expression plasmid, the luciferase activity was determined as described in Materials and Methods. Blank: different MUC5AC promoter plasmids only; control: different MUC5AC promoter plasmids plus NF-κB expression plasmid in the absence of T3; experimental: different MUC5AC promoter plasmids plus NF-κB expression plasmid in the presence of T3. 𝑃 > 0 . 0 5 versus blank; 𝑃 < 0 . 0 1 versus blank; # 𝑃 > 0 . 0 5 versus control. (c) After the cells were cotransfected with Sp1 expression plasmid and MUC −324/+48 promoter plasmid or its mutation form (mut), the luciferase activity was determined as described in Materials and Methods. Control: in the absence of T3; experimental: in the presence of T3. * 𝑃 < 0 . 0 1 versus −324/+48 control group; # 𝑃 > 0 . 0 5 versus −324/+48 mut control group.