Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation

Figure 2

Undifferentiated gene expression (Oct-4 and Nanog). AFSCs total RNA was extracted from 3 different samples by Trizol method, gene expression evaluated by RT-PCR, and electrophoresed and visualized under ultraviolet light in 2% agarose gel stained with ethidium bromide.