Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation

Figure 3

AFSCs membrane proteins were evaluated by flow cytometry with CellQuest software. Acquisitions were shown in histograms. AFSCs were positive for CD29 (98.27 ± 1.23%), CD44 (98.28 ± 1.65%), CD90 (97.69 ± 1.84%), CD105 (98.77 ± 0.64%) and were negative for CD14 (1.78 ± 1.06%), CD34 (1.70 ± 1.14%), and CD45 (1.80 ± 0.74%). IgG1-FITC and IgG1-PE antibodies were utilized as isotype controls. The number of acquired events was 20,000 in each acquisition.