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Figure 7: After thawing, AFSCs freezed with different cryoprotectants ((b) Me2SO, (c) glycerol, (d) sucrose, and (e) trehalose) were cultivated with osteogenic and adipogenic inductor medium. Differentiation was confirmed by Alizarin Red and Oil Red O staining after 21 days. (a) Control cells without inductor. Calcium deposits appear in red. All cryoprotectants tested maintained osteogenic and adipogenic differentiation potential of AFSCs after storage for 6 months.