Figure 8: AFSCs viability comparing different cryoprotectants (Me2SO, glycerol, sucrose, and trehalose) and programmed and nonprogrammed freezing protocols after 3 and 6 months. (a) viability in nonprogrammed method for 3 months; (b) viability in programmed method for 3 months; (c) viability in nonprogrammed method for 6 months; (d) viability in programmed method for 6 months. 10% Me2SO showed the higher viability rate similar to NR control in all cases. NR-AFSCs viability control measured before freezing procedure. M-Me2SO, G-glycerol, S-sucrose, and T-trehalose. Means were compared by one-way ANOVA Dunnett’s (***) and Bonferroni () post tests.