649353.fig.008a
(a)
649353.fig.008b
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649353.fig.008c
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649353.fig.008d
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Figure 8: AFSCs viability comparing different cryoprotectants (Me2SO, glycerol, sucrose, and trehalose) and programmed and nonprogrammed freezing protocols after 3 and 6 months. (a) viability in nonprogrammed method for 3 months; (b) viability in programmed method for 3 months; (c) viability in nonprogrammed method for 6 months; (d) viability in programmed method for 6 months. 10% Me2SO showed the higher viability rate similar to NR control in all cases. NR-AFSCs viability control measured before freezing procedure. M-Me2SO, G-glycerol, S-sucrose, and T-trehalose. Means were compared by one-way ANOVA Dunnett’s (*** 𝑃 < 0 . 0 5 ) and Bonferroni ( # 𝑃 < 0 , 0 5 ) post tests.