Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation

Table 2

After nonprogrammed freezing, AFSCs (𝑛=5) maintained their specific mesenchymal membrane proteins evaluated by flow cytometry (CD29, CD44, and CD105 positive; CD14, CD34, and CD45 negative). The number of acquired events was 15,000 in each acquisition. Results are shown as percentage ±SEM.

Nonprogrammed	CD14 PE	CD34 FITC	CD45 FITC	CD29 PE	CD44 FITC	CD105 PE
Me2SO 5%	1.22 ± 0.25	0.29 ± 0.25	0.35 ± 0.16	98.30 ± 1.05	99.26 ± 0.39	99.01 ± 1.03
Me2SO 10%	1.02 ± 0.18	0.74 ± 0.22	5.80 ± 2.02	99.81 ± 0.11	99.90 ± 0.07	99.26 ± 0.36
Glycerol 5%	0.94 ± 0.44	0.44 ± 0.16	0.84 ± 0.31	99.54 ± 0.26	99.91 ± 0.06	98.92 ± 0.95
Glycerol 10%	0.87 ± 0.56	0.09 ± 0.27	0.16 ± 0.09	99.10 ± 0.33	99.09 ± 0.46	99.33 ± 0.51
Sucrose 30 mM	1.06 ± 0.23	0.81 ± 0.51	1.08 ± 0.73	99.74 ± 0.04	99.69 ± 0.18	99.44 ± 0.12
Sucrose 60 mM	0.95 ± 0.65	1.14 ± 0.89	3.49 ± 0.88	99.43 ± 0.25	99.57 ± 0.32	98. 26 ± 1.39
Trehalose 60 mM	1.93 ± 0.26	0.36 ± 0.31	0.37 ± 0.26	99.86 ± 0.28	99.98 ± 0.01	98.24 ± 1.17
Trehalose 100 mM	0.82 ± 0.72	0.48 ± 0.28	0.79 ± 0.17	99.91 ± 0.05	99.08 ± 0.74	99.54 ± 0.09