Table 2: After nonprogrammed freezing, AFSCs ( 𝑛 = 5 ) maintained their specific mesenchymal membrane proteins evaluated by flow cytometry (CD29, CD44, and CD105 positive; CD14, CD34, and CD45 negative). The number of acquired events was 15,000 in each acquisition. Results are shown as percentage ±SEM.

NonprogrammedCD14 PECD34 FITCCD45 FITCCD29 PECD44 FITCCD105 PE

Me2SO 5%1.22 ± 0.250.29 ± 0.250.35 ± 0.1698.30 ± 1.0599.26 ± 0.3999.01 ± 1.03
Me2SO 10%1.02 ± 0.180.74 ± 0.225.80 ± 2.0299.81 ± 0.1199.90 ± 0.0799.26 ± 0.36
Glycerol 5%0.94 ± 0.440.44 ± 0.160.84 ± 0.3199.54 ± 0.2699.91 ± 0.0698.92 ± 0.95
Glycerol 10%0.87 ± 0.560.09 ± 0.270.16 ± 0.0999.10 ± 0.3399.09 ± 0.4699.33 ± 0.51
Sucrose 30 mM1.06 ± 0.230.81 ± 0.511.08 ± 0.7399.74 ± 0.0499.69 ± 0.1899.44 ± 0.12
Sucrose 60 mM0.95 ± 0.651.14 ± 0.893.49 ± 0.8899.43 ± 0.2599.57 ± 0.3298. 26 ± 1.39
Trehalose 60 mM1.93 ± 0.260.36 ± 0.310.37 ± 0.2699.86 ± 0.2899.98 ± 0.0198.24 ± 1.17
Trehalose 100 mM0.82 ± 0.720.48 ± 0.280.79 ± 0.1799.91 ± 0.0599.08 ± 0.7499.54 ± 0.09