Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation

Table 3

After programmed freezing, AFSCs (𝑛=5) maintained their specific mesenchymal membrane proteins evaluated by flow cytometry (CD29, CD44, and CD105 positive; CD14, CD34, and CD45 negative). The number of acquired events was 15,000 in each acquisition. Results are shown as percentage ±SEM.

Programmed	CD14 PE	CD34 FITC	CD45 FITC	CD29 PE	CD44 FITC	CD105 PE
Me2SO 5%	1.88 ± 0.35	0.58 ± 0.22	0.85 ± 0.53	96.06 ± 2.61	97.03 ± 2.06	98.82 ± 1.06
Me2SO 10%	0.92 ± 0.46	0.75 ± 0.13	0.76 ± 0.44	99.67 ± 0.16	99.88 ± 0.10	99.02 ± 0.15
Glycerol 5%	0.91 ± 0.74	0.18 ± 0.08	0.39 ± 0.29	99.48 ± 0.40	99.85 ± 0.16	99.51 ± 0.17
Glycerol 10%	1.76 ± 0.96	1.07 ± 0.97	1.12 ± 1.01	99.71 ± 0.16	99.87 ± 0.08	99.11 ± 0.39
Sucrose 30 mM	0.62 ± 0.48	0.22 ± 0.14	0.48 ± 0.15	99.29 ± 0.19	99.95 ± 0.03	99.65 ± 0.06
Sucrose 60 mM	1.36 ± 0.08	0.96 ± 0.06	1.51 ± 0.83	99.35 ± 0.28	99.17 ± 0.66	99.02 ± 0.20
Trehalose 60 mM	1.25 ± 0.41	0.31 ± 0.25	0.48 ± 0.25	97.77 ± 1.31	99.85 ± 0.11	98.90 ± 0.66
Trehalose 100 mM	0.53 ± 0.88	0.46 ± 0.36	0.60 ± 0.56	99.50 ± 0.36	99.83 ± 0.18	98.94 ± 0.76