Table 3: After programmed freezing, AFSCs ( 𝑛 = 5 ) maintained their specific mesenchymal membrane proteins evaluated by flow cytometry (CD29, CD44, and CD105 positive; CD14, CD34, and CD45 negative). The number of acquired events was 15,000 in each acquisition. Results are shown as percentage ±SEM.

ProgrammedCD14 PECD34 FITCCD45 FITCCD29 PECD44 FITCCD105 PE

Me2SO 5%1.88 ± 0.350.58 ± 0.220.85 ± 0.5396.06 ± 2.6197.03 ± 2.0698.82 ± 1.06
Me2SO 10%0.92 ± 0.460.75 ± 0.130.76 ± 0.4499.67 ± 0.1699.88 ± 0.1099.02 ± 0.15
Glycerol 5%0.91 ± 0.740.18 ± 0.080.39 ± 0.2999.48 ± 0.4099.85 ± 0.1699.51 ± 0.17
Glycerol 10%1.76 ± 0.961.07 ± 0.971.12 ± 1.0199.71 ± 0.1699.87 ± 0.0899.11 ± 0.39
Sucrose 30 mM0.62 ± 0.480.22 ± 0.140.48 ± 0.1599.29 ± 0.1999.95 ± 0.0399.65 ± 0.06
Sucrose 60 mM1.36 ± 0.080.96 ± 0.061.51 ± 0.8399.35 ± 0.2899.17 ± 0.6699.02 ± 0.20
Trehalose 60 mM1.25 ± 0.410.31 ± 0.250.48 ± 0.2597.77 ± 1.3199.85 ± 0.1198.90 ± 0.66
Trehalose 100 mM0.53 ± 0.880.46 ± 0.360.60 ± 0.5699.50 ± 0.3699.83 ± 0.1898.94 ± 0.76