657942.fig.003a
(a)
657942.fig.003b
(b)
657942.fig.003c
(c)
657942.fig.003d
(d)
Figure 3: RBC adherence efficiency and rates of erythrophagocytosis exhibited by ANeo, ANeoBro1, and ANeoADH112 trophozoites. (a-b) Trophozoites were incubated with RBC (1 : 100) at 4°C for adherence and (c-d) at 37°C for phagocytosis, for different times. RBC were contrasted with DAB and counted in 100 randomly selected trophozoites under the light microscope to obtain the mean of RBC adhered or ingested by trophozoite. Bars represent the mean ± standard error of at least three independent experiments performed by duplicate.