657942.fig.005a
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657942.fig.005b
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Figure 5: Ultrastructural location of EhADH112 in ANeoBro1 trophozoites. Ultrathin sections of ANeo or ANeoBro1 trophozoites were first prepared by cryosubstitution and incubated with pαEhADH112 and gold-labeled secondary antibodies, and then contrasted and analyzed by TEM. (a) ANeoBro1 trophozoites only incubated with gold-labeled secondary antibodies. (b–e) EhADH112-immunogold labeling in (b) plasma membrane (PM), (c) a structure resembling a MVB, (d) a lysosome-like organelle, (e) MVB and vesicles fusing to a lysosome. V: vacuole. MVB: multivesicular bodies. Arrows: vesicular fusion areas. Arrowheads: EhADH112 gold-labeled molecules.