Structure and Functional Characteristics of Rat’s Left Ventricle Cardiomyocytes under Antiorthostatic Suspension of Various Duration and Subsequent Reloading
Table 1
Transversal stiffness (pN/nm) of isolated rat’s Triton-treated left ventricular myocytes in liquid in relaxed, calcium activated (pCa = 4.2) and rigor states under gravitational unloading and subsequent reloading.
State
Group
Relaxed
Activated (pCa = 4.2)
Rigor
Transversal stiffness of the half-sarcomere area
Control ()
7.1 ± 0.4
11.0 ± 0.5@
13.2 ± 0.5@
1-HS ()
6.8 ± 0.3
10.3 ± 0.3@
12.9 ± 0.3@
3-HS ()
8.2 ± 0.3*
13.8 ± 0.4*/@
14.7 ± 0.3*/@
7-HS ()
8.9 ± 0.3*
13.5 ± 0.3*/@
15.3 ± 0.3*/@
14-HS ()
8.7 ± 0.4*
13.6 ± 0.4*/@
15.6 ± 0.4*/@
14-HS + 3-R ()
4.3 ± 0.3*/$
6.8 ± 0.3*/@/$
8.2 ± 0.5*/@/$
14-HS + 7-R ()
7.7 ± 0.3
11.0 ± 0.9@
13.4 ± 0.6@
Transversal stiffness of the M-band area
Control
9.9 ± 0.6
15.0 ± 0.6@
16.4 ± 0.5@
1-HS
8.9 ± 0.5
14.1 ± 0.4@
15.8 ± 0.5@
3-HS
9.3 ± 0.5
14.8 ± 0.6@
16.3 ± 0.5@
7-HS
10.6 ± 0.4
14.9 ± 0.5@
16.5 ± 0.8@
14-HS
9.8 ± 0.5
14.2 ± 0.6@
15.7 ± 0.3@
14-HS + 3-R
6.2 ± 0.3*/$
12.6 ± 0.4*/@/$
13.1 ± 0.4*/@/$
14-HS + 7-R
9.3 ± 0.4
14.3 ± 0.8@
17.1 ± 0.5@
Transversal stiffness of the Z-disk area
Control
16.0 ± 1.3
22.5 ± 1.5@
24.5 ± 0.9@
1-HS
15.4 ± 0.6
21.6 ± 0.3@
24.8 ± 0.6@
3-HS
15.9 ± 0.6
23.4 ± 0.7@
26.1 ± 1.3@
7-HS
15.9 ± 0.5
21.7 ± 0.3@
25.9 ± 0.6@
14-HS
15.8 ± 0.8
21.9 ± 0.9@
22.9 ± 0.7@
14-HS + 3-R
7.5 ± 0.4*/$
13.4 ± 0.5*/@/$
16.0 ± 0.4*/@/$
14-HS + 7-R
15.4 ± 0.6
21.4 ± 0.5@
23.4 ± 1.4@
as compared to the analogous state of the group “Control”, @ as compared to the relaxed state at the same group, $ as compared to the analogous state of the group “14-HS”.
