Table 2: Transversal stiffness (pN/nm) of isolated rat’s permeabilized left ventricular myocytes in liquid in relaxed, calcium activated (pCa = 4.2) and rigor states under gravitational unloading and subsequent reloading.

State
GroupRelaxedActivation (pCa = 4.2)Rigor

Transversal stiffness of sarcolemma between the M-band and Z-disk projections 𝑘 s
Control ( 𝑛 = 1 6 5 )4.03 ± 0.119.1 ± 0.4@9.67 ± 0.24@
1-HS ( 𝑛 = 1 5 3 )4.80 ± 0.22*9.5 ± 0.5@10.1 ± 0.3@
3-HS ( 𝑛 = 1 4 9 )6.22 ± 0.29*10.2 ± 0.3*/@10.8 ± 0.4*/@
7-HS ( 𝑛 = 1 4 8 )7.79 ± 0.12*10.5 ± 0.3*/@13.3 ± 0.4*/@
14-HS ( 𝑛 = 1 5 7 )12.3 ± 0.4*12.9 ± 0.4*13.3 ± 0.3*/@
14-HS + 3-R ( 𝑛 = 1 6 2 )6.4 ± 0.5*/$7.5 ± 0.3*/$8.4 ± 0.6*/$/@
14-HS + 7-R ( 𝑛 = 1 5 1 )4.3 ± 0.48.9 ± 0.5@9.2 ± 0.3@

Transversal stiffness of sarcolemma at the M-band projection 𝑘 M l s
Control2.85 ± 0.126.8 ± 0.5@7.4 ± 0.6@
1-HS2.68 ± 0.176.6 ± 0.3@7.0 ± 0.3@
3-HS3.77 ± 0.25*6.7 ± 0.4@7.7 ± 0.5@
7-HS5.79 ± 0.19*6.9 ± 0.3@7.9 ± 0.3@
14-HS7.55 ± 0.13*7.9 ± 0.6*9.4 ± 0.3*/@
14-HS + 3-R3.57 ± 0.20*/$5.9 ± 0.4$/@6.3 ± 0.3$/@
14-HS + 7-R3.1 ± 0.36.2 ± 0.4@7.0 ± 0.6@

Transversal stiffness of sarcolemma at the Z-disk projection 𝑘 Z d s
Control10.0 ± 0.314.5 ± 1.2@15.3 ± 0.5@
1-HS10.7 ± 0.815.4 ± 0.3@16.3 ± 0.5@
3-HS10.6 ± 0.314.8 ± 0.3@15.2 ± 0.7@
7-HS10.4 ± 0.311.9 ± 0.3@16.5 ± 0.3@
14-HS17.2 ± 0.4*17.9 ± 0.4*18.2 ± 0.5*/@
14-HS + 3-R9.2 ± 0.3*/$10.3 ± 0.6*/$11.9 ± 0.5*/$/@
14-HS + 7-R9.9 ± 0.715.1 ± 0.8@15.6 ± 0.4@

𝑃 < 0 . 0 5 as compared to the analogous state of the group “Control”, @ 𝑃 < 0 . 0 5 as compared to the relaxed state at the same group, $ 𝑃 < 0 . 0 5 as compared to the analogous state of the group “14-HS”.