Alterations in Cell Cycle and Induction of Apoptotic Cell Death in Breast Cancer Cells Treated with α-Mangostin Extracted from Mangosteen Pericarp
Figure 6
Expression of PCNA, cyclin D1 and p21cip1 proteins after α-mangostin treatment. Immunofluorescence of PCNA (a)–(f), cyclin D1 (g)–(l), and p21cip1 (m)–(r) in MDA-MB231 cells after 24 h of treatment with α-mangostin ((d)–(f), (j)–(l), (p)–(r)), as compared with controls ((a)–(c), (g)–(i), (m)–(o)). Yellow arrowheads in panels (c), (i), and (r) indicate higher expression of each protein in nuclei. PCNA and cyclin D1 expression was reduced and p21cip1 expression were elevated in α-mangostin-treated cells, as compared to controls. Scale bars = 25 μm.
