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Figure 2: Expression of miRNA589 in HMrSV5 cells following exposure toTGFβ1 (5 ng/ml, 0–48 hr), as assessed by realtime PCR. The bar graphs show the 2 Δ Δ C T value of miRNA589 relative to that of U6 in HMrSV5 cells following exposure to TGFβ1 (5 ng/ml, 0–48 hr). Expression of miRNA589 decreased in a time-dependent manner in HMrSV5 cells treated with TGFβ1 (column 2, 3, 4). The 2 Δ Δ C T of miRNA589 expression level of HPMCs treated with TGF-β1 for 24 h and 48 h were 0.4610 ± 0.01608 (P < 0.05) and 0.3187 ± 0.06036 (P < 0.05) as the mean ratio ± SD, respectively. ΔP > 0.05 versus control group, *P < 0.05 versus control group.