Structural Characterization and Antioxidative Activity of Low-Molecular-Weights Beta-1,3-Glucan from the Residue of Extracted Ganoderma lucidum Fruiting Bodies

Figure 6

LMG reduced H₂O₂-induced intracellular ROS levels. RAW264.7 cells were treated with H₂O₂ (20 𝜇M) and LMG (100 𝜇g/mL) for 30 min, and intracellular ROS levels were measured using DCFDA fluorescent staining and fluorescence microscopy. Left panels, untreated control; middle panels, H₂O₂ added alone; right panels, co-treatment with H₂O₂ and LMG. Images in the upper panels were captured using bright-field microscopy (original magnification 200x), and those in the lower panels were captured using fluorescence microscopy.