Table 1: Target genes of hsa-miR-548 gene family enrichment pathway analysis.

PathwayCount (gene no.)Enrichment 𝑃 valueTarget genes

Regulation of actin cytoskeleton14 2 . 1 0 𝐸 0 7 VAV2;IQGAP2;RRAS2;ITGB1;PAK7;SOS1;DOCK1;PPP1CB;SSH2;BAIAP2;ABI2;APC;ITGB3;SSH1
MAPK signaling pathway14 3 . 3 3 𝐸 0 6 DUSP4;PRKCB;RPS6KA3;RRAS2;BDNF;MAP3K2;SOS1;ELK4;TRAF6;STK4;CACNB4;MAP3K13;FOS;PRKCA
Ubiquitin mediated proteolysis13 6 . 7 7 𝐸 0 9 UBE2E3;CDC27;RCHY1;UBA2;FBXW7;HERC3;UBR5;UBE2D1;TRAF6;HERC4;UBE2B;VHL;MID1
Focal adhesion12 4 . 0 4 𝐸 0 6 VAV2;PRKCB;COL1A1;VASP;ITGB1;PAK7;SOS1;DOCK1;PPP1CB;PTEN;ITGB3;PRKCA
Melanogenesis11 2 . 7 3 𝐸 0 8 CREBBP;PRKCB;FZD3;FZD9;CALM2;KITLG;WNT5A;CREB1;CALM1;FZD7;\linebreak PRKCA
Phosphatidylinositol signaling system10 1 . 4 0 𝐸 0 8 PRKCB;CDS2;CALM2;ITPKB;DGKB;PTEN;CDS1;INPP5E;CALM1;PRKCA
Tight junction10 3 . 4 3 𝐸 0 6 LLGL2;PRKCB;RRAS2;PPP2R2B;VAPA;PARD6B;PTEN;TJP1;PPP2R2A;PRKCA
Wnt signaling pathway10 9 . 2 4 𝐸 0 6 NFAT5;CREBBP;PRKCB;TBL1X;FZD3;FZD9;WNT5A;FZD7;APC;PRKCA
Calcium signaling pathway10 4 . 7 9 𝐸 0 5 ERBB3;PRKCB;TRPC1;CALM2;ITPKB;ATP2B4;ERBB4;CALM1;SLC8A1;PRKCA
Cell cycle9 8 . 4 1 𝐸 0 6 CCNA2;CREBBP;YWHAZ;CDC27;YWHAE;CDK6;CCNB1;CHEK2;MCM6
Axon guidance9 1 . 7 2 𝐸 0 5 EPHA3;NFAT5;EPHB1;EFNB2;ITGB1;PAK7;DCC;SEMA6D;ABLIM3
Insulin signaling pathway9 2 . 7 6 𝐸 0 5 PCK1;IRS2;CALM2;SOS1;PPARGC1A;PPP1CB;RHOQ;PRKAR2B;CALM1
p53 signaling pathway8 1 . 0 8 𝐸 0 6 CCNG2;RCHY1;CDK6;PPM1D;PTEN;CCNB1;CHEK2;APAF1
Long-term potentiation8 2 . 0 5 𝐸 0 6 CREBBP;PRKCB;RPS6KA3;CALM2;PPP1CB;CALM1;GRIA1;PRKCA
Colorectal cancer8 4 . 8 5 𝐸 0 6 APPL1;FZD3;FZD9;SOS1;DCC;FZD7;APC;FOS
Glioma7 8 . 4 3 𝐸 0 6 PRKCB;CALM2;SOS1;CDK6;PTEN;CALM1;PRKCA
ErbB signaling pathway7 5 . 7 3 𝐸 0 5 ERBB3;EREG;PRKCB;PAK7;SOS1;ERBB4;PRKCA
Glycosphingolipid biosynthesis-lactoseries6 3 . 6 5 𝐸 0 7 B4GALT4;B3GALT2;B4GALT1;FUT9;ST8SIA1;GCNT2
Nonsmall cell lung cancer6 3 . 1 4 𝐸 0 5 RASSF5;PRKCB;SOS1;CDK6;STK4;PRKCA
B cell receptor signaling pathway6 2 . 0 1 𝐸 0 4 VAV2;NFAT5;PRKCB;SOS1;DAPP1;FOS
Adherens junction6 2 . 6 7 𝐸 0 4 CREBBP;LMO7;SSX2IP;TJP1;BAIAP2;PTPRB
TGF-beta signaling pathway6 4 . 5 0 𝐸 0 4 ACVR2B;BMPR1A;CREBBP;SMAD7;BMPR2;BMPR1B
GnRH signaling pathway60.001PRKCB;MAP3K2;CALM2;SOS1;CALM1;PRKCA
Leukocyte transendothelial migration60.002RASSF5;VAV2;PRKCB;VASP;ITGB1;PRKCA
Natural killer cell mediated cytotoxicity60.005VAV2;NFAT5;PRKCB;SOS1;SH2D1A;SH2D1A;PRKCA
Cytokine-cytokine receptor interaction60.076ACVR2B;BMPR1A;TNFSF13B;KITLG;BMPR2;BMPR1B
Basal cell carcinoma5 3 . 7 9 𝐸 0 4 FZD3;FZD9;WNT5A;FZD7;APC
Cholera-infection5 5 . 2 6 𝐸 0 4 PRKCB;TJP1;ATP6V0A2;KDELR2;PRKCA
Adipocytokine signaling pathway5 9 . 4 3 𝐸 0 4 ACSL6;PCK1;IRS2;PPARGC1A;ACSL5
PPAR signaling pathway50.001ACSL6;PCK1;FABP3;ME1;ACSL5
ECM-receptor interaction50.003CD47;COL1A1;DAG1;ITGB1;ITGB3
Gap junction50.004PRKCB;MAP3K2;SOS1;TJP1;PRKCA
T cell receptor signaling pathway50.008VAV2;NFAT5;PAK7;SOS1;FOS
Renal cell carcinoma50.001CREBBP;EPAS1;PAK7;SOS1;EPAS1;VHL
Small cell lung cancer50.003ITGB1;CDK6;TRAF6;PTEN;APAF1
Alzheimer’s disease50.049CALM2;UQCRB;CALM1;APAF1;NDUFA4

Here, we only list important pathways that involve at least 5 target mRNAs of hsa-miR-548 gene family.