Production of Chemoenzymatic Catalyzed Monoepoxide Biolubricant: Optimization and Physicochemical Characteristics

Table 2

D-optimal design arrangement and responses for MEOA.

Run no.	H2O2 (X1)	Catalyst^a (X2)	Time^b (X3)	Y1, yield (%)	Y2,  OOC (%)	RCO (%)	Y3, IV (mg/g)	X (%)	SE
1	20	80	8	76.57	6.17	68.40	37.81	76.37	0.89
2	17.5	80	7	88.57	5.48	60.75	58.95	62.53	0.97
3	17.5	100	8	72.14	7.54	83.59	32.22	79.52	1.05
4	20	80	6	72.28	6.4	70.95	40.98	73.95	0.95
5	20	120	7	54.71	5.94	65.85	64.32	59.12	1.11
6	20	120	6	60.28	7.88	87.36	30.87	80.38	1.08
7	15	120	8	73.57	5.48	60.75	53.24	66.16	0.91
8	20	80	7	81.42	5.37	59.53	56.32	64.20	0.92
9	15	100	7	75.68	5.02	55.65	74.64	52.56	1.05
10	18.75	100	7	85.28	5.71	63.30	49.17	68.75	0.92
11	20	100	7	81.14	6.05	67.06	42.72	72.85	0.92
12	15	120	6	70.78	4.57	50.66	76.48	51.39	0.98
13	15	100	6	65.93	3.65	40.46	96.43	38.71	1.00
14	15	120	7	82.14	4.91	54.43	66.65	57.64	0.94
15	17.5	120	8	59.28	6.74	74.72	36.37	76.63	0.97
16	20	100	6	72.85	6.51	72.17	39.76	74.73	0.90
17	15	80	8	77.14	4.34	48.11	83.85	46.71	1.03
18	17.5	100	6	80.85	3.77	41.79	87.09	44.65	0.93

Notes: OOC, oxirane oxygen content; RCO, relative percentage conversion to oxirane; IV, iodine value; X, conversion to double bond; SE, oxirane oxygen selectivity.
^aCatalyst Novozym 435 (mg).
^bMonoepoxidation time (h).