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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 695843, 11 pages
http://dx.doi.org/10.1155/2012/695843
Research Article

Plasmodium Riboprotein PfP0 Induces a Deviant Humoral Immune Response in Balb/c Mice

1Malarial Parasite Biology Laboratory, Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai 400005, India
2Bioklone, Chennai 600061, India
3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Centre, Johns Hopkins Medical Institute, Baltimore, MD 21231, USA
4Department of Medicine, SCB Medical College Hospital, Cuttack 753007, India
5Basic and Clinical Immunology of Parasitic diseases Group, Centre for Infection and Immunity of Lille, 59019 Lille Cedex, France
6Infectious Disease Biology Group, Institute of Life Sciences, Bhubaneswar 751023, India

Received 5 July 2011; Revised 30 September 2011; Accepted 2 October 2011

Academic Editor: Jorge Morales-Montor

Copyright © 2012 Sulabha Pathak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Passive immunization with antibodies to recombinant Plasmodium falciparum P0 riboprotein (rPfP0, 61–316 amino acids) provides protection against malaria. Carboxy-terminal 16 amino acids of the protein (PfP0C0) are conserved and show 69% identity to human and mouse P0. Antibodies to this domain are found in 10–15% of systemic lupus erythematosus patients. We probed the nature of humoral response to PfP0C0 by repeatedly immunizing mice with rPfP0. We failed to raise stable anti-PfP0C0 hybridomas from any of the 21 mice. The average serum anti-PfP0C0 titer remained low (5.1±1.3×104). Pathological changes were observed in the mice after seven boosts. Adsorption with dinitrophenyl hapten revealed that the anti-PfP0C0 response was largely polyreactive. This polyreactivity was distributed across all isotypes. Similar polyreactive responses to PfP0 and PfP0C0 were observed in sera from malaria patients. Our data suggests that PfP0 induces a deviant humoral response, and this may contribute to immune evasion mechanisms of the parasite.