Table 1: Primers used to amplify antibody variable regions.

Heavy chain 3′ primer
IgG15′ AGG CTT ACT AGT ACA ATC CCT GGG CAC AAT 3′

Heavy chain 5′ primers
HC15′ AGG TCC AGC TGC TCG AGT CTG 3′
HC25′ AGG TCC AGC TGC TCG AGT CAG 3′
HC35′ AGG TCC AGC TTC TCG AGT CTG 3′
HC45′ AGG TCC AGC TTC TCG AGT CAG 3′
HC55′ AGG TCC AAC TGC TCG AGT CTG 3′
HC65′ AGG TCC AAC TGC TCG AGT CAG 3′
HC75′ AGG TCC AAC TTC TCG AGT CTG 3′
HC85′ AGG TCC AAC TTC TCG AGT CAG 3′

Kappa light chain 3′ primer
MKC5′ GCG CCG TCT AGA ATT AAC ACT CAT TCC TGT TGA A 3′

Kappa light chain 5′ primers
LC15′ CCA GTT CCG AGC TCG TTG TGA CTC AGG AAT CT 3′
LC25′ CCA GTT CCG AGC TCG TGT TGA CGC AGC CGC CC 3′
LC35′ CCA GTT CCG AGC TCG TGC TCA CCC AGT CTC CA 3′
LC45′ CCA GTT CCG AGC TCC AGA TGA CCC AGT CTC CA 3′
LC55′ CCA GAT GTG AGC TCG TGA TGA CCC AGA CTC CA 3′
LC65′ CCA GAT GTG AGC TCG TCA TGA CCC AGT CTC CA 3′
LC75′ CCA GTT CCG AGC TCG TGA TGA CAC AGT CTC CA 3′