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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 716023, 7 pages
http://dx.doi.org/10.1155/2012/716023
Research Article

Assessment of Genotoxic and Cytotoxic Hazards in Brain and Bone Marrow Cells of Newborn Rats Exposed to Extremely Low-Frequency Magnetic Field

Department of Biophysics, Faculty of Science, Cairo University, Giza 12013, Egypt

Received 23 May 2012; Accepted 10 August 2012

Academic Editor: Brynn Levy

Copyright © 2012 Monira M. Rageh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study aimed to evaluate the association between whole body exposure to extremely low frequency magnetic field (ELF-MF) and genotoxic , cytotoxic hazards in brain and bone marrow cells of newborn rats. Newborn rats (10 days after delivery) were exposed continuously to 50 Hz, 0.5 mT for 30 days. The control group was treated as the exposed one with the sole difference that the rats were not exposed to magnetic field. Comet assay was used to quantify the level of DNA damage in isolated brain cells. Also bone marrow cells were flushed out to assess micronucleus induction and mitotic index. Spectrophotometric methods were used to measure the level of malondialdehyde (MDA) and the activity of glutathione (GSH) and superoxide dismutase (SOD). The results showed a significant increase in the mean tail moment indicating DNA damage in exposed group ( 𝑃 < 0 . 0 1 , 0 . 0 0 1 , 0 . 0 0 0 1 ). Moreover ELF-MF exposure induced a significant ( 𝑃 < 0 . 0 1 , 0 . 0 0 1 ) four folds increase in the induction of micronucleus and about three folds increase in mitotic index ( 𝑃 < 0 . 0 0 0 1 ). Additionally newborn rats exposed to ELF-MF showed significant higher levels of MDA and SOD ( 𝑃 < 0 . 0 5 ). Meanwhile ELF-MF failed to alter the activity of GSH. In conclusion, the present study suggests an association between DNA damage and ELF-MF exposure in newborn rats.