Table 3: Profile of inflammatory mediators in different phases of DSS-induced colitis in mice.

Tissue/methodAnalytes measuredacute DSS colitischronic DSS colitisMouse strainref.

7 days of DSS treatment

Colon/RT PCRIL-1, IL-4, IL-10, IL-12, IFNγ, TNFα↑ IL-1, IL-10, IL-12,
↑ IFN-γ, TNFα
BALB/c[42]

5 days of DSS treatment 5 days of DSS followed by 28 days of water

Colon/ELISA, MSIIL-1α, IL-1β, IL-12 p40, IL-12 p70, IL-17, IL-6, G-CSF, IL-18↑ IL-1α, IL-1β, IL-12 p40,
↑ IL-12 p70, IL-17, IL-6,
↑ G-CSF
↑ IL-1α, IL-1β, IL-12 p40,
↑ IL-12 p70, IL-17, IL-6,
↑ G-CSF
C57BL/6J[22]
↑ IL-1α, IL-1β, IL-6,
↑ IL-18, G-CSF
↑ IL-12 p40BALB/c[22]

5 days of DSS treatment 5 days of DSS followed by 7 days of water

Colon/RT PCRTNF-α, IL-1β, IL-6, IL-10, IL-12, IFN-γ, KC, MIP-2↑ TNF-α, IL-1β, IL-6,
↑ IL-10, IL-12, IFN-γ,
↑ KC, MIP-2
↑ TNF-α, IL-1β, IL-6, IL-10,
↑ IL-12, IFN-γ, KC, MIP-2
C57BL/6J[39]

7 days of DSS treatment4 cycles of 7days of DSS followed by 10 days of water

Serum/MSIIL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-17, IFN-γ, TNF-α, GM-CSF, IP-10, KC, MCP-1, MIG, MIP-1α↑ IL-6, IL-17, TNF-α, KC↑ IL-6, IFN-γ, IL-4, IL-10[43]
Colon/Western IL-6, IL-12, IL-23, IL-17, IFN-γ↑ IL-6, IFN-γ, IL-17↑ IL-6C57BL/6J[43]

RT PCR: real-time PCR; MSI: multiplex sandwich immunoassay; IFN-γ: interferone gama; IL: interleukine; GM-CSF: granulocyte-macrophage colony-stimulating factor; IP-10: interferon-inducible protein; KC: keratinocyte-derived chemokine; MCP-1: monocyte chemoattractant protein; MIG: monokine induced by IFN-γ; MIP-1α: macrophage inflammatory protein; TNF-α: tumor necrosis factor alpha.