Table 1: Apoptosis (percentage of cells) of DLA cells treated with deoxyelephantopin as determined by microscopic morphological observation, stained with acridine orange and ethidium bromide.

Con. of deoxyelephantopin (μg/mL)Treatment time (6 h)Treatment time (18 h)
Percentage of cellsx (%)Percentage of cellsx (%)
Viable cellsNon-viable cellsViable cellsNon-viable cells
ApoptoticNecroticApoptoticNecrotic

0 (control) 9 5 ± 4 . 2 5 ± 0 . 2 5 9 2 ± 4 . 5 2 ± 0 . 2 8 6 ± 0 . 4 9
1 6 8 ± 3 . 8 2 6 ± 1 . 7 1 6 ± 0 . 4 0 6 1 ± 4 . 0 2 3 0 ± 2 . 1 5 9 ± 0 . 5 6
3 4 5 ± 2 . 1 5 3 9 ± 2 . 7 9 1 6 ± 1 . 7 2 2 7 ± 1 . 9 3 4 6 ± 3 . 2 9 2 7 ± 1 . 8 5
6 2 9 ± 1 . 7 8 3 0 ± 1 . 6 8 4 1 ± 2 . 5 6 2 0 ± 1 . 3 7 3 5 ± 2 . 4 4 5 ± 4 . 0 4
8 1 5 ± 0 . 8 7 9 ± 0 . 5 2 7 6 ± 5 . 2 2 1 3 ± 1 . 1 7 5 ± 0 . 4 5 8 2 ± 5 . 1 2
10 1 2 ± 0 . 6 7 9 ± 0 . 5 5 7 9 ± 4 . 8 6 1 0 ± 0 . 9 0 6 ± 0 . 3 7 8 4 ± 7 . 5 5

xcells (viable + nonviable) = 100%, *** 𝑃 < 0 . 0 0 1 compared to control.