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721657.fig.0010b
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Figure 10: Overexpression of Plg-RKT increases cell surface plasminogen activation. PC12 cells stably overexpressing Plg-RKT (pCIneo-Plg-RKT) (a) or vector alone (pCIneo) (b) were analyzed by dual color FACS analysis. Viable cells were gated from nonviable cells and histogram plots of viable cells are shown. Dotted tracings: anti-Plg-RKT mAb IgG. Black tracings: isotype control IgG. Dashed tracings: autofluorescence. (c) PC12 cells stably overexpressing Plg-RKT (pCIneo-Plg-RKT) or vector alone (pCIneo) were incubated with plasminogen and t-PA was added and plasminogen activation was measured. Cell-mediated plasminogen activation was substantially greater in Plg-RKT overexpressing cells than in control cells ( 𝑃 < 0 . 0 0 1 at each cell concentration tested) ( 𝑛 = 3 ). [25]. ©The American Society for Biochemistry and Molecular Biology.