721657.fig.008a
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721657.fig.008b
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Figure 8: Plg-RKT re-directs GFP to the cell membrane. PC12 cells were transiently transfected with pAcGFP-Plg-RKT (in which the Plg-RKT cDNA was inserted in frame for expression of a GFP-Plg-RKT fusion protein, with Plg-RKT at the C-terminus) or with control pAcGFP vector. (a) Cell lysates were Western blotted with polyclonal anti-Plg-RKT IgG (lanes 1 and 2) or anti-GFP IgG (lanes 3 and 4). (b) The transfected cells were stained with a combination of DAPI and WGA-rhodamine. The first and third panels of images represent maximum projections of a series of optical slices through the cells. The GFP-Plg-RKT signal was localized primarily to the plasma membrane. The second and fourth panels of images represent sagittal (apical-basal) slices through the same cells along the white dotted line indicated. Here the peripheral plasma membrane localization of GFP-Plg-RKT is also evidentthroughout the vertical stacks of images that were acquired. Scale bar in bottom right corner of image represents 10 microns. [25]. ©The American Society for Biochemistry and Molecular Biology.