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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 730462, 10 pages
http://dx.doi.org/10.1155/2012/730462
Research Article

Genistein Attenuates Vascular Endothelial Impairment in Ovariectomized Hyperhomocysteinemic Rats

1Department of Pathology, The Luhe Teaching Hospital of the Capital Medical University, 82 Xinhuanan Road, Tongzhou District, Beijing 101149, China
2Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, You An Men Wai, Fengtai District, Beijing 100069, China
3The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Beijing An Zhen Hospital, Capital Medical University, Ministry of Education, 2 Anzhen Road, Chaoyang District, Beijing 100029, China
4Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, Dongcheng District, Beijing 100005, China

Received 27 March 2012; Revised 14 October 2012; Accepted 15 October 2012

Academic Editor: Paul Higgins

Copyright © 2012 Panpan Zhen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. Ross, “The pathogenesis of atherosclerosis: a perspective for the 1990s,” Nature, vol. 362, no. 6423, pp. 801–809, 1993. View at Publisher · View at Google Scholar · View at Scopus
  2. C. Antoniades, A. S. Antonopoulos, D. Tousoulis, K. Marinou, and C. Stefanadis, “Homocysteine and coronary atherosclerosis: from folate fortification to the recent clinical trials,” European Heart Journal, vol. 30, no. 1, pp. 6–15, 2009. View at Publisher · View at Google Scholar · View at Scopus
  3. G. N. Welch and J. Loscalzo, “Homocysteine and atherothrombosis,” The New England Journal of Medicine, vol. 338, no. 15, pp. 1042–1050, 1998. View at Publisher · View at Google Scholar · View at Scopus
  4. P. Y. Chang, S. C. Lu, C. M. Lee et al., “Homocysteine inhibits arterial endothelial cell growth through transcriptional downregulation of fibroblast growth factor-2 involving G protein and DNA methylation,” Circulation Research, vol. 102, no. 8, pp. 933–941, 2008. View at Publisher · View at Google Scholar · View at Scopus
  5. R. C. Austin, S. R. Lentz, and G. H. Werstuck, “Role of hyperhomocysteinemia in endothelial dysfunction and atherothrombotic disease,” Cell Death and Differentiation, vol. 11, supplement 1, pp. S56–S64, 2004. View at Publisher · View at Google Scholar · View at Scopus
  6. G. S. Hossain, J. V. van Thienen, G. H. Werstuck et al., “TDAG51 is induced by homocysteine, promotes detachment-mediated programmed cell death, and contributes to the development of atherosclerosis in hyperhomocysteinemia,” The Journal of Biological Chemistry, vol. 278, no. 32, pp. 30317–30327, 2003. View at Publisher · View at Google Scholar · View at Scopus
  7. J. C. Chambers, A. McGregor, J. Jean-Marie, O. A. Obeid, and J. S. Kooner, “Demonstration of rapid onset vascular endothelial dysfunction after hyperhomocysteinemia: an effect reversible with vitamin C therapy,” Circulation, vol. 99, no. 9, pp. 1156–1160, 1999. View at Scopus
  8. P. M. Kanani, C. A. Sinkey, R. L. Browning, M. Allaman, H. R. Knapp, and W. G. Haynes, “Role of oxidant stress in endothelial dysfunction produced by experimental hyperhomocyst(e)inemia in humans,” Circulation, vol. 100, no. 11, pp. 1161–1168, 1999. View at Scopus
  9. R. T. Eberhardt, M. A. Forgione, A. Cap et al., “Endothelial dysfunction in a murine model of mild hyperhomocyst(e)inemia,” Journal of Clinical Investigation, vol. 106, no. 4, pp. 483–491, 2000. View at Scopus
  10. N. Tyagi, K. C. Sedoris, M. Steed, A. V. Ovechkin, K. S. Moshal, and S. C. Tyagi, “Mechanisms of homocysteine-induced oxidative stress,” American Journal of Physiology, vol. 289, no. 6, pp. H2649–H2656, 2005. View at Publisher · View at Google Scholar · View at Scopus
  11. V. E. R. Edirimanne, C. W. H. Woo, Y. L. Siow, G. N. Pierce, J. Y. Xie, and O. Karmin, “Homocysteine stimulates NADPH oxidase-mediated superoxide production leading to endothelial dysfunction in rats,” Canadian Journal of Physiology and Pharmacology, vol. 85, no. 12, pp. 1236–1247, 2007. View at Publisher · View at Google Scholar · View at Scopus
  12. S. E. Walker, M. R. Adams, A. A. Franke, and T. C. Register, “Effects of dietary soy protein on iliac and carotid artery atherosclerosis and gene expression in male monkeys,” Atherosclerosis, vol. 196, no. 1, pp. 106–113, 2008. View at Publisher · View at Google Scholar · View at Scopus
  13. U. Wenzel, D. Fuchs, and H. Daniel, “Protective effects of soy-isoflavones in cardiovascular disease: identification of molecular targets,” Hamostaseologie, vol. 28, no. 1-2, pp. 85–88, 2008. View at Scopus
  14. S. E. Walker, T. C. Register, S. E. Appt et al., “Plasma lipid-dependent and -independent effects of dietary soy protein and social status on atherogenesis in premenopausal monkeys: implications for postmenopausal atherosclerosis burden,” Menopause, vol. 15, no. 5, pp. 950–957, 2008. View at Publisher · View at Google Scholar · View at Scopus
  15. H. Si and D. Liu, “Phytochemical genistein in the regulation of vascular function: new insights,” Current Medicinal Chemistry, vol. 14, no. 24, pp. 2581–2589, 2007. View at Publisher · View at Google Scholar · View at Scopus
  16. G. Rimbach, C. Boesch-Saadatmandi, J. Frank et al., “Dietary isoflavones in the prevention of cardiovascular disease—a molecular perspective,” Food and Chemical Toxicology, vol. 46, no. 4, pp. 1308–1319, 2008. View at Publisher · View at Google Scholar · View at Scopus
  17. D. Fuchs, B. Dirscherl, J. H. Schroot, H. Daniel, and U. Wenzel, “Soy extract has different effects compared with the isolated isoflavones on the proteome of homocysteine-stressed endothelial cells,” Molecular Nutrition and Food Research, vol. 50, no. 1, pp. 58–69, 2006. View at Publisher · View at Google Scholar · View at Scopus
  18. D. Fuchs, S. de Pascual-Teresa, G. Rimbach et al., “Proteome analysis for identification of target proteins of genistein in primary human endothelial cells stressed with oxidized LDL or homocysteine,” European Journal of Nutrition, vol. 44, no. 2, pp. 95–104, 2005. View at Publisher · View at Google Scholar · View at Scopus
  19. D. Fuchs, B. Dirscherl, J. H. Schroot, H. Daniel, and U. Wenzel, “Proteome analysis suggests that mitochondrial dysfunction in stressed endothelial cells is reversed by a soy extract and isolated isoflavones,” Journal of Proteome Research, vol. 6, no. 6, pp. 2132–2142, 2007. View at Publisher · View at Google Scholar · View at Scopus
  20. H. Si and D. Liu, “Isoflavone genistein protects human vascular endothelial cells against tumor necrosis factor-alpha-induced apoptosis through the p38beta mitogen-activated protein kinase.,” Apoptosis, vol. 14, no. 1, pp. 66–76, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. D. Fuchs, P. Erhard, G. Rimbach, H. Daniel, and U. Wenzel, “Genistein blocks homocysteine-induced alterations in the proteome of human endothelial cells,” Proteomics, vol. 5, no. 11, pp. 2808–2818, 2005. View at Publisher · View at Google Scholar · View at Scopus
  22. K. Mahn, C. Borrás, G. A. Knock et al., “Dietary soy isoflavone induced increases in antioxidant and eNOS gene expression lead to improved endothelial function and reduced blood pressure in vivo,” The FASEB Journal, vol. 19, no. 12, pp. 1755–1757, 2005. View at Publisher · View at Google Scholar · View at Scopus
  23. J. Kolling, E. B. Scherer, A. A. da Cunha, M. J. da Cunha, and A. T. S. Wyse, “Homocysteine induces oxidative-nitrative stress in heart of rats: prevention by folic acid,” Cardiovascular Toxicology, vol. 11, no. 1, pp. 67–73, 2011. View at Publisher · View at Google Scholar · View at Scopus
  24. N. Weiss, “Mechanisms of increased vascular oxidant stress in hyperhomocysteinemia and its impact on endothelial function,” Current Drug Metabolism, vol. 6, no. 1, pp. 27–36, 2005. View at Publisher · View at Google Scholar · View at Scopus
  25. R. F. Furchgott and J. V. Zawadzki, “The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine,” Nature, vol. 288, no. 5789, pp. 373–376, 1980. View at Scopus
  26. C. R. de Andrade, S. Y. Fukada, V. C. Olivon et al., “α1D-adrenoceptor-induced relaxation on rat carotid artery is impaired during the endothelial dysfunction evoked in the early stages of hyperhomocysteinemia,” European Journal of Pharmacology, vol. 543, no. 1–3, pp. 83–91, 2006. View at Publisher · View at Google Scholar · View at Scopus
  27. V. S. Mujumdar, G. M. Aru, and S. C. Tyagi, “Induction of oxidative stress by homocyst(e)ine impairs endothelial function,” Journal of Cellular Biochemistry, vol. 82, no. 3, pp. 491–500, 2001. View at Publisher · View at Google Scholar · View at Scopus
  28. M. Hansrani and G. Stansby, “The use of an in vivo model to study the effects of hyperhomocysteinaemia on vascular function,” Journal of Surgical Research, vol. 145, no. 1, pp. 13–18, 2008. View at Publisher · View at Google Scholar · View at Scopus
  29. F. L. Zheng, J. H. Zhao, and H. P. Zhang, “The action of PI3K/AKT during genistein promoting the activity of eNOS,” Zhonghua Xin Xue Guan Bing Za Zhi, vol. 40, no. 4, pp. 327–331, 2012.
  30. X. Yu, X. Cheng, J. J. Xie et al., “Poly (ADP-ribose) polymerase inhibition improves endothelial dysfunction induced by hyperhomocysteinemia in rats,” Cardiovascular Drugs and Therapy, vol. 23, no. 2, pp. 121–127, 2009. View at Publisher · View at Google Scholar · View at Scopus
  31. H. Y. Cho, C. M. Park, M. J. Kim, R. Chinzorig, C. W. Cho, and Y. S. Song, “Comparative effect of genistein and daidzein on the expression of MCP-1, eNOS, and cell adhesion molecules in TNF-α-stimulated HUVECs,” Nutrition Research and Practice, vol. 5, no. 5, pp. 381–388, 2011. View at Publisher · View at Google Scholar · View at Scopus
  32. H. Si, J. Yu, H. Jiang, H. Lum, and D. Liu, “Phytoestrogen genistein up-regulates endothelial nitric oxide synthase expression via activation of cAMP response element-binding protein in human aortic endothelial cells,” Endocrinology, vol. 153, no. 7, pp. 3190–3198, 2012. View at Publisher · View at Google Scholar · View at Scopus
  33. S. Wassmann, K. Wassmann, and G. Nickenig, “Modulation of oxidant and antioxidant enzyme expression and function in vascular cells,” Hypertension, vol. 44, no. 4, pp. 381–386, 2004. View at Publisher · View at Google Scholar · View at Scopus
  34. J. Vásquez-Vivar, J. Whitsett, P. Martásek, N. Hogg, and B. Kalyanaraman, “Reaction of tetrahydrobiopterin with superoxide: EPR-kinetic analysis and characterization of the pteridine radical,” Free Radical Biology and Medicine, vol. 31, no. 8, pp. 975–985, 2001. View at Publisher · View at Google Scholar · View at Scopus
  35. M. Yanagisawa, H. Kurihara, S. Kimura et al., “A novel potent vasoconstrictor peptide produced by vascular endothelial cells,” Nature, vol. 332, no. 6163, pp. 411–415, 1988. View at Scopus
  36. M. Sattler, S. Verma, G. Shrikhande et al., “The BCR/ABL tyrosine kinase induces production of reactive oxygen species in hematopoietic cells,” The Journal of Biological Chemistry, vol. 275, no. 32, pp. 24273–24278, 2000. View at Publisher · View at Google Scholar · View at Scopus
  37. J. Yang, L. J. Wu, S. I. Tashino, S. Onodera, and T. Ikejima, “Protein tyrosine kinase pathway-derived ROS/NO productions contribute to G2/M cell cycle arrest in evodiamine-treated human cervix carcinoma HeLa cells,” Free Radical Research, vol. 44, no. 7, pp. 792–802, 2010. View at Publisher · View at Google Scholar · View at Scopus
  38. K. J. Dammanahalli and Z. Sun, “Endothelins and NADPH oxidases in the cardiovascular system,” Clinical and Experimental Pharmacology and Physiology, vol. 35, no. 1, pp. 2–6, 2008. View at Publisher · View at Google Scholar · View at Scopus
  39. M. Iglarz and M. Clozel, “Mechanisms of ET-1-induced endothelial dysfunction,” Journal of Cardiovascular Pharmacology, vol. 50, no. 6, pp. 621–628, 2007. View at Publisher · View at Google Scholar · View at Scopus
  40. J. Zhang, V. M. Narayan, N. Juedes, and J. M. Patel, “Hypoxic upregulation of preproendothelin-1 gene expression is associated with protein tyrosine kinase-PI3K signaling in cultured lung vascular endothelial cells,” International Journal of Clinical and Experimental Medicine, vol. 2, no. 1, pp. 87–94, 2009. View at Scopus