AngiotensinII Preconditioning Promotes Angiogenesis In Vitro via ERKs Phosphorylation
Figure 1
Time-dependent effects of AngII preconditioning on AngII-induced impairment of capillary-like tube formation in cultured CMVECs. CMVECs were pretreated with AngII (10−7 M) for the indicated times and then seeded onto the matrigel in plates containing AngII (10−6 M) or not (control). Eighteen hrs later, the formation of capillary-like tubes was observed under an optical microscope. (a) Representative photomicrographs of capillary-like tubes (100xmagnification). (b) Quantitative analysis for capillary-like tube formation. Capillary-like tubes were counted in randomly selected 5 fields for each plate. Data represent mean ± SD obtained from 15 independent experiments (); versus control group; versus the group without AngII pretreatment (0 min).
