AngiotensinII Preconditioning Promotes Angiogenesis In Vitro via ERKs Phosphorylation
Figure 4
Effects of ERKs inhibition on AngII preconditioning-induced improvement of vasculature formation under the condition of AngII stimulation. CMVECs preincubated with or without PD98059 () for 30 min were treated with 10−7 M AngII for the indicated times, and then the cells were seeded onto the matrigel in plates containing AngII (10−6 M) or not (control). Eighteen hrs later, the formation of capillary-like tubes was observed under an optical microscope. (a) Representative photomicrographs of capillary-like tubes (100xmagnification). (b) Quantitative analysis for capillary-like tube formation. Capillary-like tubes were counted in randomly selected 5 fields for each plate. Data represent mean ± SD obtained from 12 independent experiments (). versus control group; versus the group without AngII pretreatment (0 min); ▲P versus the group pretreated with AngII (10 min) but without PD98059.
