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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 741542, 8 pages
http://dx.doi.org/10.1155/2012/741542
Methodology Report

Technical Considerations for Reduced Representation Bisulfite Sequencing with Multiplexed Libraries

1Department of Pathology, Dunedin School of Medicine, University of Otago, 270 Great King Street, Dunedin 9054, New Zealand
2National Research Centre for Growth and Development, 2-6 Park Avenue, Grafton, Auckland 1142, New Zealand
3Department of Biochemistry, University of Otago, 710 Cumberland Street, Dunedin 9054, New Zealand

Received 19 June 2012; Accepted 18 September 2012

Academic Editor: Wolfgang Arthur Schulz

Copyright © 2012 Aniruddha Chatterjee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary material contains- schematic of barcoded adaptor ligation to MspI mitigated fragments (Figure S1), Bioanalyzer quality trace of the RRBS library (Figure S2), Purification gel of smaller fragments (Figure S3), Example of different data format generated by various sequencer versions (Figure S4). Feature comparison of Illumina GAII/Genome Analyser and HiSeq2000 sequencers (Table S1), Comparison of overall library preparation workflow between Illumina Paired-End and TruSeq protocols (Table S2), Step-wise recipe of multiplexed adaptor ligation of RRBS libraries (Table S3) and Recipe of PCR amplification step of the indexed RRBS libraries (Table S4).

  1. Supplementary Material