Methodology Report
Technical Considerations for Reduced Representation Bisulfite Sequencing with Multiplexed Libraries
Table 1
Comparison between base calling performed by RTA (real-time analyzer) and OLB (off-line basecaller) of multiplexed samples.
| | Sample ID | Number of bases after RTA | Number of bases after OLB | Percentage of change (RTA versus OLB) | Number of reads after RTA | Number of reads after OLB | Percentage of change (RTA versus OLB) |
| | Read1 | | | | | | | | 1 |
1520286198
|
1548285294
|
1.8
|
16377421
|
16503971
|
0.8
| | 2 |
2282620124
|
2350814836
|
3.0
|
24702835
|
25201462
|
2.0
| | 3 |
2753391480
|
2846632920
|
3.4
|
30043201
|
30847092
|
1.7
| | 4 |
1280388372
|
1325584268
|
3.5
|
13754015
|
14152462
|
2.9
| | 5 |
1837282806
|
1881566740
|
2.4
|
20849236
|
21178885
|
1.6
|
| | Read2 | | | | | | | | 1 |
1512562937
|
1503732114
| −0.7 |
16377421
|
16503971
|
0.8
| | 2 |
2214536843
|
2212529249
| −0.1 |
24702835
|
25200712
|
2.0
| | 3 |
2621636705
|
2631071280
|
0.4
|
30043201
|
30847092
|
2.7
| | 4 |
1265479416
|
1276840465
|
0.9
|
13754015
|
14152462
|
2.9
| | 5 |
1516530411
|
1492797800
| −1.6 |
20849236
|
21178885
|
1.6
|
|
|
