Table 1: Comparison between base calling performed by RTA (real-time analyzer) and OLB (off-line basecaller) of multiplexed samples.

Sample IDNumber of bases after RTANumber of bases after OLBPercentage of change (RTA versus OLB)Number of reads after RTANumber of reads after OLBPercentage of change (RTA versus OLB)

Read1
 1 1520286198 1548285294 1.8 16377421 16503971 0.8
 2 2282620124 2350814836 3.0 24702835 25201462 2.0
 3 2753391480 2846632920 3.4 30043201 30847092 1.7
 4 1280388372 1325584268 3.5 13754015 14152462 2.9
 5 1837282806 1881566740 2.4 20849236 21178885 1.6

Read2
 1 1512562937 1503732114 −0.7 16377421 16503971 0.8
 2 2214536843 2212529249 −0.1 24702835 25200712 2.0
 3 2621636705 2631071280 0.4 30043201 30847092 2.7
 4 1265479416 1276840465 0.9 13754015 14152462 2.9
 5 1516530411 1492797800 −1.6 20849236 21178885 1.6