Technical Considerations for Reduced Representation Bisulfite Sequencing with Multiplexed Libraries

Table 3

Comparison of mapping performance between RTA and OLB datasets.

Sample ID	RTA		OLB
	Unique mapping (%)	Uniquely aligned reads	Unique mapping (%)	Uniquely aligned reads
1	71.2	11513092	71.9	11681235
2	59.4	13998639	58.3	13945432
3	66.5	18785673	55.3	15940503
4	71.4	9603345	71.9	9907812
5	63.4	11296038	63.8	11508956

¹The mapping runs were performed on a Mac Pro with 64 bit duo quad core Intel Xeon processors and with 22 Gb RAM running Mac OS 10.6. The samples were mapped using Bismark v0.6.4 against the GRCh37.65 build of the human genome.