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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 742829, 8 pages
http://dx.doi.org/10.1155/2012/742829
Research Article

Expression and Cellular Distribution of INHA and INHB before and after In Vitro Cultivation of Porcine Oocytes Isolated from Follicles of Different Size

1Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland
2Department of Anatomy, Poznań University of Medical Sciences, 6 Święcickiego St., 60-781 Poznań, Poland
3Department of Veterinary, Poznań University of Life Sciences, 52 Wojska Polskiego St., 60-628 Poznań, Poland
4Department of Toxicology, Poznań University of Medical Sciences, 30 Dojazd St., 60-631 Poznań, Poland
5Department of Reproductive Biology, Leibniz Institute for Farm Animal Biology, Wilhelm-Stahl Allee 2, 18196 Dummerstorf, Germany

Received 24 July 2012; Revised 8 October 2012; Accepted 23 October 2012

Academic Editor: Leon Spicer

Copyright © 2012 Bartosz Kempisty et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cumulus-oocyte-complexes (COCs) were collected from small (<3 mm), medium (3–5 mm), and large (>5 mm) porcine follicles, and the INHA and INHB expression and cellular localization were studied. Developmentally competent (BCB+) COCs were cultured for 44 h. Samples of mRNA were isolated before and after in vitro maturation (IVM) from oocytes collected from follicles of different size for RQ-PCR assay. The INHA and INHB protein distribution within the oocytes was observed by confocal microscopy. INHA mRNA expression was increased in oocytes from large compared to medium and small follicles before IVM ( ), and to oocytes of small follicles after IVM ( ). The INHB expression was not different before IVM, but the IHNB mRNA level was gradually higher in oocytes from large follicles after IVM ( ). INHA was not differently expressed before IVM; however, in large follicle oocytes the protein was distributed in the peripheral area of the cytoplasm; in oocytes from small follicles it was in the entire cytoplasm. After IVM, INHA was strongly expressed in oocytes from small follicles and distributed particularly in the zona pellucida (ZP). Similarly and both before and after IVM, INHB protein was highly expressed in small follicle oocytes and within the cytoplasm. In summary, INHs can be recognized as a marker of porcine oocyte quality.