742829.fig.003a
(a)
742829.fig.003b
(b)
742829.fig.003c
(c)
742829.fig.003d
(d)
742829.fig.003e
(e)
742829.fig.003f
(f)
Figure 3: Confocal microscopic observation of INHB cellular distribution in porcine oocytes isolated from small, medium, and large follicles before and after IVM. Porcine oocytes isolated from small, medium, and large follicles were stained before and after IVM with porcine INHB (before IVM; (a), (b), and (c) and after IVM; (d), (e), and (f)), (rabbit polyclonal anti-INHB, sc-50288). The treated oocytes were labeled for 40 min with TRITC (crystalline tetramethylrhodamine isothiocyanate), which emits a red fluorescent signal after excitation at 543 nm at a 1 : 200 dilution in PBS for INHB. Bars are 50 μm. L: large, M: medium, S: small follicles.