Camel Milk Modulates the Expression of Aryl Hydrocarbon Receptor-Regulated Genes, Cyp1a1, Nqo1, and Gsta1, in Murine hepatoma Hepa 1c1c7 Cells

Figure 4

Effect of camel milk on the TCDD-mediated induction of Cyp1a1 protein. Hepa 1c1c7 cells were treated for 24 with TCDD (1 nM) in the presence and absence of camel milk (fat-free) (0, 25, and 100 𝜇L/mL); thereafter, Cyp1a1 protein level was determined by Western blot analysis and was detected using the enhanced chemiluminescence method. The intensity of Cyp1a1 protein bands was quantified relative to the signals obtained for Gapdh protein, using ImageJ. One of the three representative experiments is shown and values represent mean of fold change ± SEM. (𝑛=3). ⁺𝑃<0.05 compared with control (sterile water-treated cells), ^∗𝑃<0.05 compared with TCDD-treated cells.