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Journal of Biomedicine and Biotechnology
Volume 2012 (2012), Article ID 805418, 7 pages
http://dx.doi.org/10.1155/2012/805418
Research Article

Maternal Moderate Physical Training during Pregnancy Attenuates the Effects of a Low-Protein Diet on the Impaired Secretion of Insulin in Rats: Potential Role for Compensation of Insulin Resistance and Preventing Gestational Diabetes Mellitus

1Department of Nutrition, Federal University of Pernambuco, 50670-901 Recife, PE, Brazil
2Department of Physical Education and Sports Science, CAV, Federal University of Pernambuco, 55608-680 Recife, PE, Brazil
3Department of Neuropsychiatry and Behavioral Science, Federal University of Pernambuco, 50670-420 Recife, PE, Brazil
4Institute of Biomedical Science, University of São Paulo, 05508-000 São Paulo, SP, Brazil
5Institute of Physical Activity Sciences and Sports, Cruzeiro do Sul University, 01506-000 São Paulo, SP, Brazil

Received 14 June 2012; Revised 16 July 2012; Accepted 18 July 2012

Academic Editor: Renata Gorjão

Copyright © 2012 Carol Góis Leandro et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The effects of pregestational and gestational low-to-moderate physical training on insulin secretion in undernourished mothers were evaluated. Virgin female Wistar rats were divided into four groups as follows: control (C, 𝑛 = 5 ); trained (T, 𝑛 = 5 ); low-protein diet (LP, 𝑛 = 5 ); trained with a low-protein diet (T + LP, 𝑛 = 5 ). Trained rats ran on a treadmill over a period of 4 weeks before mate (5 days week−1 and 60 min day−1, at 65% of V O 2 m a x ). At pregnancy, the intensity and duration of the exercise were reduced. Low-protein groups were provided with an 8% casein diet, and controls were provided with a 17% casein diet. At third day after delivery, mothers and pups were killed and islets were isolated by collagenase digestion of pancreas and incubated for a further 1 h with medium containing 5.6 or 16.7 mM glucose. T mothers showed increased insulin secretion by isolated islets incubated with 16.7 mM glucose, whereas LP group showed reduced secretion of insulin by isolated islets when compared with both C and LP + T groups. Physical training before and during pregnancy attenuated the effects of a low-protein diet on the secretion of insulin, suggesting a potential role for compensation of insulin resistance and preventing gestational diabetes mellitus.