Lim Mineralization Protein 3 Induces the Osteogenic Differentiation of Human Amniotic Fluid Stromal Cells through Kruppel-Like Factor-4 Downregulation and Further Bone-Specific Gene Expression
Figure 3
qPCR gene expression analysis. The graphic represents the expression levels of osteospecific gene ((a) BMP2; (b) RunX2; (c) SMARCC2; (d) OC; (e) OSX; (f) OP). Values represent the mean of triplicate experiments for each time point tested. AdΨ5: mock-transduced control cells; AdLMP-3: treated cells; OM: cells cultivated in osteogenic medium as positive controls; d: days after cell transduction (4d-time point was not assessed in OM-cells); *: statistical significance, . Fold change was calculated according to the 2‒ΔΔCt method.
